Effects of Treadmill Running on Extracellular Basal Levels of Glutamate and Gaba at Dentate Gyrus of Streptozotocin-induced Diabetic Rats

BACKGROUND: The present study evaluated the effects of treadmill running on extracellular basal levels of glutamate and GABA at dentate gyrus of streptozotocin-induced diabetic rats.

JRMS 2010; 15(3): 172-174 lthough diabetes mellitus impairs learning and memory, 1 the mechanism of these impairments is not been well understood and treatment with insulin reversed them partially. 2It has been demonstrated that diabetes affects synthesis and release of neurotransmitters that are involved in learning and memory in hippocampus such as glutamate. 3ue to the fact that regular physical exercise has beneficial effects on neural health and function in diabetes, [4][5][6] the main objective of this study was to determine the effects of streptozotocin-induced diabetes and treadmill running on basal levels of glutamate and GABA in dentate gyrus of rats.

Methods
The subjects were male wistar rats (230 ± 20 g) that were divided into four groups (n = 6-7): the control-rest, the control-exercise, the diabetes-rest and the diabetes-exercise.To induce diabetes, streptozotocin (60 mg/kg in saline i.p.) was given to each animal.Animals with blood glucose levels higher than 300 mg/dl after 3 days were selected. 5Exercise protocol was daily treadmill running at a speed of 17 m/min for 40 minutes for 12 weeks at 0° of inclination.
After 12 weeks of diabetes induction and exercise duration, rats were anesthetized with urethane (1.8 g/kg i.p.) 5 and through stereotaxic surgery a microdialysis probe (dialysis A www.SID.ir

A r c h i v e o f S I D
Exercise alleviate diabetic neural complications Reisi et al membrane of 1 mm in length) was placed in the dentate gyrus and using a microdialysis pump the probes were perfused with artificial cerebrospinal fluid at a rate of 2 µl/min and the dialysates were collected for 1 hour.Measurement was made by reverse-phase high pressure liquid chromatography coupled to fluorescence detection, following pre-column derivatization with o-phthaladialdehyde. 3 Data were analyzed statistically using oneway ANOVA followed by Tukey's test.The significant level was set at p < 0.05.Results are expressed as mean ± SEM.

Results
The blood glucose concentrations at the end of experiments are shown in table 1.
As it is shown in figure 1, glutamate levels were significantly decreased in diabetes-rest group comparing to the control-rest (p < 0.05) and the diabetes-exercise groups (p < 0.05); however, there were no significant differences between the other groups.In addition GABA levels had no significant differences between the groups.

Discussion
This study reported that diabetes reduces basal levels of dentate gyrus glutamate but not GABA, and that impairments are attenuated following exercise.
Although exercise seems to have both preventive and therapeutic effects on the defects of brain functions in diabetes, the underlying mechanisms are poorly understood.Possible explanations are: Exercise prevents suppression of cell proliferation that is produced by diabetes in the dentate gyrus. 4 In diabetes, hyperglycemia decreases extracellular gulutamate 7 ; however studies have demonstrated that exercise lowers hyperglycemia (Table 1). 8xercise increases the expression of hippocampal neurotrophic factors 9 that are decreased in diabetes. 10

Conclusions
The data correspond to the possibility that treadmill running is helpful in alleviating neurotransmitter homeostasis and alterations in transmission in diabetes mellitus.

Figure 1 .
Figure 1.Effects of STZ-induced diabetes and exercise on the extracellular glutamate (a) and GABA (b) content of the microdialysate from the dentate gyrus of anesthetized rats after 12 weeks of diabetes induction and exercise duration.Data are expressed as mean ± SEM. (n = 6-7) * P < 0.05 with respect to the control group † P < 0.05 with respect to the diabetes-exercise group

Table 1 .
Blood glucose concentrations at the end of experiments